TitleCellulose-based chromatography for cellooligosaccharide production.
Publication TypeJournal Article
Year of Publication2004
AuthorsAkpinar, O, McGorrin, RJ, Penner, MH
JournalJ Agric Food Chem
Volume52
Issue13
Pagination4144-8
Date Published2004 Jun 30
ISSN0021-8561
KeywordsCellulose, Chemical Fractionation, Chemical Phenomena, Chemistry, Physical, Chromatography, Chromatography, High Pressure Liquid, Crystallization, Drug Stability, Ethanol, Hot Temperature, Oligosaccharides
Abstract

The potential of using cellulose stationary phases for the chromatographic fractionation of cellooligosaccharide preparations has been explored. The impetus for the work is the current interest in using cellooligosaccharides as functional nondigestible oligosaccharides in foods. The conceptual studies illustrate the potential of using ethanol-water mobile phases in conjunction with cellulose stationary phases for cellooligosaccharide fractionation. Cellooligosaccharide solubility in ethanol-water mixtures and their elution order from cellulose-based columns using ethanol-water mobile phases were shown to be in line with their degree of polymerization (DP), with the higher DP cellooligosaccharides being less soluble and having longer retention times. The retention volume for all COS increased with increased temperature. Both microcrystalline and fibrous cellulose preparations were shown to work as chromatographic stationary phases. The application experiments demonstrate the potential of using cellulose stationary phases for the cleanup and fractionation of cellooligosaccharide mixtures generated via acid-catalyzed hydrolysis of cellulose.

DOI10.1021/jf035305m
Alternate JournalJ. Agric. Food Chem.
PubMed ID15212461