TitleComparison of sugar, acids, and volatile composition in raspberry bushy dwarf virus-resistant transgenic raspberries and the wild type 'meeker' (rubus idaeus L.).
Publication TypeJournal Article
Year of Publication2008
AuthorsMalowicki, SMM, Martin, R, Qian, MC
JournalJ Agric Food Chem
Volume56
Issue15
Pagination6648-55
Date Published2008 Aug 13
ISSN1520-5118
KeywordsCarbohydrates, Fruit, Gas Chromatography-Mass Spectrometry, Hydrogen-Ion Concentration, Odorants, Plant Diseases, Plants, Genetically Modified, RNA Viruses, Rosaceae, Volatilization
Abstract

Raspberry bushy dwarf virus (RBDV) causes a significant reduction in yield and quality in raspberry and raspberry-blackberry hybrid. Genetic modifications were made to 'Meeker' red raspberries to impart RBDV resistance. The RBDV-resistant transgenic and wild type 'Meeker' plants were grown in Oregon and Washington, and the fruits were harvested in the 2004 and 2005 growing seasons. Year-to-year and site-to-site variations were observed for the degrees Brix and titratable acidity, with Oregon raspberries having slightly higher degrees Brix and lower titratable acidity than Washington raspberries. Twenty-nine volatile compounds were quantified using stir bar sorptive extraction (SBSE) paired with gas chromatography-mass spectrometry (GC-MS). There were very few differences in volatile concentrations between the transgenic varieties and the wild type 'Meeker'. Much larger variations were observed between sites and harvest seasons. Raspberries grown in Oregon appeared to have higher concentrations of delta-octalactone, delta-decalactone, geraniol, and linalool. Chiral analysis of alpha-ionone, alpha-pinene, linalool, terpinen-4-ol, delta-octalactone, and delta-decalactone demonstrated a much higher percentage of one isomer over the other, particularly alpha-ionone, alpha-pinene, delta-octalactone, and delta-decalactone, with more than 90% of one isomer, while a racemic mixture was observed for linalool. The isomeric analysis revealed very little variation between varieties, locations, or years. The flavor compounds tested in this study did not show any difference between the transgenic lines and the wild type 'Meeker' raspberry.

DOI10.1021/jf800253e
Alternate JournalJ. Agric. Food Chem.
PubMed ID18598047