TitleDevelopment of cytotoxicity tests for assessment of the toxicity of water samples taken from the environment.
Publication TypeJournal Article
Year of Publication1987
AuthorsBenford, DJ, Good, SP
JournalMol Toxicol
Volume1
Issue4
Pagination419-25
Date Published1987-1988 Fall
ISSN0883-9492
KeywordsAnimals, Cell Line, Cell Survival, Cricetinae, Lethal Dose 50, Rats, Toxicology, Water Pollutants, Water Pollutants, Chemical
Abstract

Different methods of measuring cytotoxicity have been investigated in order to establish a test system for assessment of the toxicity of water samples. Ideally this should be highly sensitive and rapid to perform. Four variations of the neutral red uptake test have been compared with the MTT test and ATP determination. Chinese hamster V79/4 cells were used as the test system with 10 model toxins. All tests gave essentially similar results, with linear regression analysis producing correlation coefficients in excess of .93. No single test was most sensitive to all 10 compounds. The neutral red uptake and MTT tests could be performed in a single working day (approximately 8 h) if test compound and cells were plated out simultaneously. These tests are preferable to ATP determination, which is a complex and lengthy procedure, requiring expensive reagents.

Alternate JournalMol Toxicol
PubMed ID3509694