TitleEnhancement of chicken macrophage cytokine response to Salmonella Typhimurium when combined with bacteriophage P22.
Publication TypeJournal Article
Year of Publication2013
AuthorsPark, SHong, Biswas, D, Lingbeck, J, Koo, OKyung, Ricke, SC
JournalFEMS Microbiol Lett
Volume339
Issue2
Pagination137-44
Date Published2013 Feb
ISSN1574-6968
KeywordsAnimals, Bacteriophage P22, Cell Line, Chickens, Enzyme-Linked Immunosorbent Assay, Interleukin-8, Macrophages, Real-Time Polymerase Chain Reaction, Salmonella typhimurium
Abstract

Salmonella infections are reported as the second most common pathogen caused foodborne disease in the United States, and several Salmonella serovars can colonize in the intestinal tracts of poultry. Reducing Salmonella in poultry is crucial to decrease the incidence of salmonellosis in humans. In this study, we evaluated the immune response of chicken macrophage cells (HD-11) and effects of bacteriophage P22 against the extra- and intracellular S. Typhimurium LT2. Four treatments, (1) HD-11 cells as control, (2) HD-11 cells with LT2, (3) HD-11 cells with LT2 and P22, and (4) HD-11 cells with P22, were administered, and IL-8 responses of HD-11 cells were measured using an ELISA. Also, four cytokine (IL-4, IL-8, IL-10, and IFN-γ) gene expression levels in the presence of LT2 and/or P22 were quantified by qRT-PCR. We found that P22 lysed the extra- and intracellular LT2, which adhered and were taken up by the HD-11 cells. The ELISA indicated that HD-11 cells produced significantly higher IL-8 cytokine levels in the supernatant during the intracellular lyses of LT2 by P22 (P < 0.05). The IL-8 expression levels measured by qRT-PCR also exhibited similar results with the IL-8 production based on ELISA measurements.

DOI10.1111/1574-6968.12064
Alternate JournalFEMS Microbiol. Lett.
PubMed ID23240769