TitleHSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status.
Publication TypeJournal Article
Year of Publication1998
AuthorsWells, DR, Tanguay, RL, Le, H, Gallie, DR
JournalGenes Dev
Volume12
Issue20
Pagination3236-51
Date Published1998 Oct 15
ISSN0890-9369
KeywordsAmino Acid Sequence, Cell Respiration, Eukaryotic Initiation Factor-3, Eukaryotic Initiation Factor-4G, Heat-Shock Proteins, Molecular Sequence Data, Peptide Initiation Factors, Plant Proteins, Plants, Toxic, Poly A, Protein Biosynthesis, Proto-Oncogene Proteins, RNA, Messenger, Tobacco, Transcription Factors, Triticum
Abstract

The 5' leader (Omega) of tobacco mosaic viral RNA functions as a translational enhancer. Sequence analysis of a 102-kD protein, identified previously as a specific Omega RNA-binding protein, revealed homology to the HSP101/HSP104/ClpB family of heat shock proteins and its expression in yeast complemented a thermotolerance defect caused by a deletion of the HSP104 gene. Up to a 50-fold increase in the translation of Omega-luc, but not luc mRNA was observed in yeast expressing the tobacco HSP101 whereas Omega failed to enhance translation in the absence of HSP101. Therefore, HSP101 and Omega comprise a two-component translational regulatory mechanism that can be recapitulated in yeast. Analysis of HSP101 function in yeast translation mutants suggested that the initiation factor (eIF) 3 and specifically one (TIF4632) of the two eIF4G proteins were required for the HSP101-mediated enhancement. The RNA-binding and translational regulatory activities of HSP101 were inactive in respiring cells or in cells subject to nutrient limitation, but its thermotolerance function remained unaffected. This is the first identification of a protein required for specific translational enhancement of capped mRNAs, the first report of a translational regulatory function for any heat-shock protein, and the first functional distinction between the two eIF4G proteins present in eukaryotes.

Alternate JournalGenes Dev.
PubMed ID9784498
PubMed Central IDPMC317219