TitleImmuno-based detection of Shiga toxin-producing pathogenic Escherichia coli in food - A review on current approaches and potential strategies for optimization.
Publication TypeJournal Article
Year of Publication2016
AuthorsBaker, CA, Rubinelli, PM, Park, SHong, Ricke, SC
JournalCrit Rev Microbiol
Volume42
Issue4
Pagination656-75
Date Published2016 Aug
ISSN1549-7828
KeywordsFlow Cytometry, Food Microbiology, Immunoassay, Shiga-Toxigenic Escherichia coli
Abstract

Certain pathogenic Escherichia coli known as Shiga toxin (Stx)-producing E. coli (STEC) are a public health threat to the consumer, and are problematic for the food industry. Food products containing STEC are deemed unfit for human consumption, and STEC illnesses can cause hemolytic uremic syndrome (HUS), a disease affecting the kidneys in susceptible individuals. Optimizing detection methods in foods have been focused on more prompt and accurate analysis. This review addresses the role and applications of immuno-based assays for STEC detection in food systems. Immunoassay antibody capture systems and flow cytometry platforms have been implemented into several food-based detection systems. By applying antibodies that will interact with target microorganisms, immunoassays can be used to directly detect and quantify pathogens. Immuno-based protocols could potentially be further implemented into the food industry, limit the duration of the detection process and increase accuracy.

DOI10.3109/1040841X.2015.1009824
Alternate JournalCrit. Rev. Microbiol.
PubMed ID26016737