TitleMicrobial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains.
Publication TypeJournal Article
Year of Publication2017
AuthorsPark, SHong, Kim, SAe, Rubinelli, PM, Roto, SM, Ricke, SC
JournalVaccine
Volume35
Issue24
Pagination3204-3208
Date Published2017 05 31
ISSN1873-2518
KeywordsAnimals, Cecum, Chickens, Gastrointestinal Microbiome, High-Throughput Nucleotide Sequencing, Poultry Diseases, Salmonella Infections, Animal, Salmonella typhimurium, Salmonella Vaccines
Abstract

Previously, we constructed and characterized the vaccine efficacy of Salmonella Typhimurium mutant strains in poultry with either inducible mviN expression (P-mviN) or methionine auxotrophy (ΔΔmetRmetD). The aim of the present study was to assess potential impact of these Salmonella vaccine strains on the cecal microbiota using a next generation sequencing (NGS). The cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with P-mviN; group 3, vaccinated with wild type; group 4, vaccinated with ΔΔmetRmetD) were subjected to microbiome sequencing analysis with an Illumina MiSeq platform. The NGS microbiome analysis of chicken ceca revealed considerable changes in microbial composition in the presence of the different vaccine strains and exhibited detectable patterns of distinctive clustering among the respective groups (the R value of unweighted PCoA plot was 0.68). The present study indicates that different S. Typhimurium vaccine strains can differentially influence the microbiota of the ceca in terms of presence but not in the relative abundance of microbiota.

DOI10.1016/j.vaccine.2017.04.073
Alternate JournalVaccine
PubMed ID28479173