TitleRegulated expression of virulence gene mviN provides protective immunity and colonization control of Salmonella in poultry.
Publication TypeJournal Article
Year of Publication2015
AuthorsRubinelli, PM, Lee, SIn, Roto, SM, Park, SHong, Ricke, SC
JournalVaccine
Volume33
Issue41
Pagination5365-5370
Date Published2015 Oct 05
ISSN1873-2518
KeywordsAnimals, Antibodies, Bacterial, Bacterial Proteins, Cell Line, Cell Survival, Chickens, Gene Expression Regulation, Bacterial, Immunoglobulin G, Macrophages, Male, Mutation, Poultry, Salmonella, Salmonella Infections, Animal, Time Factors, Vaccination, Virulence
Abstract

Current live attenuated vaccines for control of Salmonella in poultry persist in the ceca and may persist in the environment. In this paper we report the construction and characterization of the vaccine efficacy of a Salmonella mutant strain with inducible mviN expression and rapid clearance from the host. The mutant was effective in oral immunization of the broiler chicken host against a virulent Salmonella oral challenge strain, having a mean 7×10(6)CFU/g in the ceca of unvaccinated controls compared to a mean 2×10(3)CFU/g in the ceca of vaccinated chickens at 4 weeks post-challenge (6 weeks of age). The mutant strain also demonstrated immunogenicity, reduced organ colonization, and rapid clearance in broiler chickens within 3 weeks of inoculation.

DOI10.1016/j.vaccine.2015.08.074
Alternate JournalVaccine
PubMed ID26354731